Toll Like Receptor 2, NT (TLR2, CD282) (MaxLight 550), Rabbit
Biozol Catalog Number:
USB-T8050-17F-ML550
Supplier Catalog Number:
T8050-17F-ML550
Alternative Catalog Number:
USB-T8050-17F-ML550-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
FLISA, IHC, WB
Immunogen:
Peptide corresponding to an 14aa sequence near the N-terminus of human TLR2. Epitope: the first 50aa of TLR2.
MaxLight(TM)550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor(TM)546, 555, DyLight(TM)549 , Cy3(TM), TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm), Emission (575nm), Extinction Coefficient 150,000. Toll Like Receptor 2 (TLR2) is a 90kD type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. TLR2 is also expressed in the spleen, bone marrow and lymph nodes. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2. Applications: Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: detected a band of ~100kD in A-20 cell lysates. Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Positive Control: Human spleen Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
