Recombinant protein corresponding to Met360-Leu516 from human toll-like receptor 7, expressed in E. coli. Species Sequence Homology: mouse, 81%
Toll-like receptor 7 (TLR7) is a 120kD (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. Human TLR7 is synthesized as a 1049aa precursor that contains a 26aa signal sequence, an 803aa extracellular domain (ECD), a 21aa transmembrane sequence, and a 189aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid pre-dendritic cells. Functionally, TLR7 participates in the innate immune response to microbial agents. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells from a mouse. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
