KLH-conjugated synthetic peptide corresponding to human TLR8, within amino acids 750-850.
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR8 gene contains three exons, two of which have coding function (4). TLR8 cDNA codes for a protein of approximate molecular weight of 120kD (4,5). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-3ug/ml. Cell lysate from 293 transfected with human TLR8 was used as positive control. A protein of~20kD was detected. Flow Cytometry (intracellular): 2-5ug/1x10e6 cells. Ramos cells can be used as a positive control. Immunohistochemistry (paraffin): 5ug/ml. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
