TOLLIP, also known as Toll interacting protein, is an inhibitory adaptor protein within Toll-like receptors. The TLR pathway is a part of the innate immune system that recognizes structurally conserved molecular patterns of microbial pathogens, leading to an inflammatory immune response. Negative regulation of TLR signaling by TOLLIP may limit the production of proinflammatory mediators during inflammation and infection. Also, it forms a complex with Tom1 to regulate endosomal trafficking of ubiquitinated proteins. Recombinant human TOLLIP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified Source: Recombinant corresponding to aa1-274 from human TOLLIP, fused to His-tag at N-terminus expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MATTVSTQRG PVYIGELPQD FLRITPTQQQ RQVQLDAQAA QQLQYGGAVG TVGRLNITVV QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW NKVIHCTVPP GVDSFYLEIF DERAFSMDDR IAWTHITIPE SLRQGKVEDK WYSLSGRQGD DKEGMINLVM SYALLPAAMV MPPQPVVLMP TVYQQGVGYV PITGMPAVCS PGMVPVALPP AAVNAQPRCS EEDLKAIQDM FPNMDQEVIR SVLEAQRGNK DAAINSLLQM GEEP Molecular Weight: ~32.4kD (294aa) confirmed by MALDI-TOF Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
