DNA topoisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis. In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100kD molecular weight. Most of this precursor is then proteolytically processed to a species with 70kD molecular weight from which the Scl-70 antigen has derived its name. Recombinant Scl-70 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Expressed in Sf9 insect cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
91.58
Purity:
95% (SDS-PAGE)
Form:
Supplied as a liquid in 16mM HEPES, pH 7.5, 400mM sodium chloride, 20% glycerol
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