Scl-70, Recombinant, Human, Western Blot Positive Control (Scleroderma, DNA Topoisomerase I)
Biozol Catalog Number:
USB-T8065-02A
Supplier Catalog Number:
T8065-02A
Alternative Catalog Number:
USB-T8065-02A-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
WB
DNA topoisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis. In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100kD molecular weight. Most of this precursor is then proteolytically processed to a species with 70kD molecular weight from which the Scl-70 antigen has derived its name. Recombinant Human Scl-70 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 dalton. Recombinant Scl-70 is expressed with a 6x His tag and purified by proprietary chromatographic techniques. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Suitable for use in Western Blot. Other applications not tested. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
95%
Form:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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