Topoisomerase II (DNA) alpha, IgG2a, Clone: [2Q382], Mouse, Monoclonal
Catalog Number:
USB-T8065-11
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| Article Name: | Topoisomerase II (DNA) alpha, IgG2a, Clone: [2Q382], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-T8065-11 |
| Supplier Catalog Number: | T8065-11 |
| Alternative Catalog Number: | USB-T8065-11-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | FC, IHC, IP, WB |
| Immunogen: | Nuclear protein preparation from the human cell line U937.50 |
| The expression of topoisomerase II alpha is strongly restricted to proliferating cells. The topoisomerase II alpha antigen is preferentially expressed during G1, S, G2 and M phase of the cell cycle, while resting, non-cycling cells (G0 phase) lack topoisomerase II alpha. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase II alpha during the entire cell-cycle. This specificity for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas and gangliomas. Applications: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml Immunohistochemistry: 5-10ug/ml. Strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections. Immunocytochemistry: 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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