Topoisomerase II (DNA) alpha (Biotin), IgG2a, Clone: [2Q382], Mouse, Monoclonal
Catalog Number:
USB-T8065-11-BIOTIN
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| Article Name: | Topoisomerase II (DNA) alpha (Biotin), IgG2a, Clone: [2Q382], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-T8065-11-BIOTIN |
| Supplier Catalog Number: | T8065-11-Biotin |
| Alternative Catalog Number: | USB-T8065-11-BIOTIN-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | FC, IHC, IP, WB |
| Immunogen: | Nuclear protein preparation from the human cell line U937.50 |
| The expression of topoisomerase II alpha is strongly restricted to proliferating cells. The topoisomerase II alpha antigen is preferentially expressed during G1, S, G2 and M phase of the cell cycle, while resting, non-cycling cells (G0 phase) lack topoisomerase II alpha. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase II alpha during the entire cell-cycle. This specificity for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas and gangliomas. Applications: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml Immunohistochemistry: 5-10ug/ml. Strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections. Immunocytochemistry: 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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