TORC2 (TORC 2, TORC-2, Transducer of Regulated CREB Protein 2, CREB-regulated Transcription Coactivator 2, CRTC2, Transducer of Regulated cAMP Response Element-binding Protein), IgG2a, Clone: [11D113], Rat, Monoclonal
Biozol Catalog Number:
USB-T8067-21C
Supplier Catalog Number:
T8067-21C
Alternative Catalog Number:
USB-T8067-21C-100
Manufacturer:
US Biological
Host:
Rat
Category:
Antikörper
Application:
WB
Immunogen:
Recombinant protein corresponding to Lys454-Ser612 from mouse TORC2 expressed in E. coli.
TORC2, also known as CREB regulated transcription coactivator 1 (crtc2), is a 7080kD protein that functions as a coactivator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its Oglycosylation triggers TORC2 dependent induction of hepatic gluconeogenic enzymes. It mediates hypothalamic glucose sensing and also the sensing of endoplasmic reticulum stress. In addition, TORC2 contributes to the transcription of HTLV 1 genes and the activation of latent EBV infections. Within aa454-612 mouse TORC2 shares 84% and 98% aa sequence identity with human and rat TORC2, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml in NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and RAW 264.7 mouse monocyte/macrophage cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
