Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates and completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil, or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn. Applications: Suitable for use in Immunofluorescence, FLISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:10-1:50 Acetone fixation of the antigen source is recommended prior to staining. FLISA: 1:20-1:200 Western Blot: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[5E443]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added. Labeled with Fluorescein isothiocyanate (FITC).
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