Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates and completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil, or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Traditionally, Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[1.B.516 TP3]
Isotype:
IgG2a
Purity:
Purified by Protein G affinity chromatography. Purity tested by electrophoresis.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
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