TRAIL (TNF-Related Apoptosis Inducing Ligand, Apo2 Ligand), Clone: [4i170], Mouse, Monoclonal

Product Overview

• Article Name: TRAIL (TNF-Related Apoptosis Inducing Ligand, Apo2 Ligand), Clone: [4i170], Mouse, Monoclonal
• Biozol Catalog Number: USB-T8180-04
• Supplier Catalog Number: T8180-04
• Alternative Catalog Number: USB-T8180-04-500
• Manufacturer: US Biological
• Host: Mouse
• Category: Antikörper
• Immunogen: Recombinant protein corresponding to human TRAIL derived from E. coli.

Product Description

Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 2-4ug/ml (100ul/well) detects at least 500pg/ml of recombinant human TRAIL when used with biotinylated anti-human TRAIL (catalog 143686) as detection antibody at a concentration of 1-2ug/ml. Western Blot: 0.25-0.5ug/ml. The detection limit is 0.25-0.5ng/lane of recombinant human TRAIL, under either reducing or non-reducing conditions. Neutralization: 0.3-0.6ug/ml to yield one-half the maximal inhibition of the biological activity of human TRAIL (10ng/ml). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [4i170]
• NCBI: 003801
• UniProt: P50591
• Purity: Purified by Protein A affinity chromatography from ascites.
• Form: Supplied as a lyophilized powder from PBS. Reconstitute with 500ul of sterile ddH2O.