Transferrin receptor is a 190kD Type II membrane glycoprotein which consists of two subunits of 95kD. It is expressed on activated lymphocytes, macrophages and monocytes. It binds Fe (Apo) transferrin. The gene coding for the antigen is located on the q-arm of chromosome 3. The ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias and lymphomas. Transferrin receptors are also present on reticulocytes, but are lost during maturation into erythrocytes.Transferrin receptor (CD71) has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. The transferrin receptor has been structurally characterized as a disulphide-bound dimer of identical glycoprotein subunits of 95kDa. The transferrin receptor is not present on resting peripheral blood lymphocytes, but appears after mitogenic stimulation on B and T cells. Erythroblasts express high levels of CD71. It is also present on macrophages. It is expressed by some T-and B-cell lines. CD71 expression is linked to cell proliferation and intracellular iron concentration. Has been studied at the Vth International Workshop on Human Leukocyte Differentiation Antigens in Boston (1993). Applications: Suitable for use in FLISA and Western blot. Other applications not tested. Recommended Dilution: Optimal dilution determined by the researcher. Hybridoma: SP2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[1.BB.544]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography ( 90%).
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).
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