Transferrin Receptor, soluble, Human, BioAssay(TM) ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)

Product Overview

• Article Name: Transferrin Receptor, soluble, Human, BioAssay(TM) ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)
• Biozol Catalog Number: USB-T8199-38
• Supplier Catalog Number: T8199-38
• Alternative Catalog Number: USB-T8199-38-1
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The transferrin receptor (TfR) is the gateway for transferrin-bound-iron entering all body cells. TfR is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body TfR content. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85kD glycoprotein forming in serum a 320kD complex with diferric transferrin. The serum sTfR concentration reflects the total body mass of cellular transferrin receptor. Anaemias associated with enhanced erythropoiesis and iron deficiency result in an elevation in the sTfR values. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in its decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease. The Human sTfR BioAssay(TM) ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum and plasma (EDTA, heparin, citrate). Test Principle: Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibodysTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve. Detection Range: 0.05-2ug/ml Sensitivity: 2ng/ml Specificity: Human soluble transferrin receptor Kit Components: T8199-38A: Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab T8199-38B: Mab (HRP), 1x13ml T8199-38C: Standard, 2ug/ml, 1x100ul T8199-38D: Standard, 1ug/ml, 1x100ul T8199-38E: Standard, 0.5ug/ml, 1x.100ul T8199-38F: Standard, 0.2ug/ml, 1x100ul T8199-38G: Standard, 0.1ug/ml, 1x100ul T8199-38H: Standard, 0.05ug/ml, 1x100ul T8199-38J: Quality Control, High, 50X, 1x50ul T8199-38K: Quality Control, Low, 50X, 1x50ul T8199-38L: Dilution Buffer, 2x13ml T8199-38M: Wash Solution, 10X, 1x100ml T8199-38N: Substrate Solution (TMB), 1x13ml T8199-38P: Stop Solution, 1x13ml Storage and Stability: Store kit at 4C. Unopened kit components are stable for 6 months after receipt. Opened reagents are stable for 3 months when stored at 4C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.