Recombinant protein corresponding to human transferrin receptor
The human transferrin receptor (TfR) is a homodimeric type II membrane protein consisting of two identical 95kD subunits covalently linked by intermolecular disulfide bonds. The TfR has an extracellular domain of 671 amino acids, a single 28 residue transmembrane region and a 61 residue amino terminal cytoplasmic domain. Expression of the receptor is limited on normal tissue, however, highly expressed on the surface of some tumors. TfR is included in the class of ligand transport receptors which are internalized and clustered in coated pits. An important function of the receptor is to bind the serum transport protein transferrin (Tf) and mediate the uptake of iron into the cell. A growing area of research involves the use of monoclonal anti-TF receptor antibodies to inhibit the uptake of iron and thus block tumor cell growth. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Electron Microscopy. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[6A202]
Isotype:
IgG1
Purity:
Purified by affinity chromatography
Form:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Horseradish peroxidase (HRP).
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