Applications: Detection of transferrin in serum by sandwich FLISA. Detection of transferrin-transferrin receptor complex by sandwich FLISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with placental transferrin receptor or transferrin. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[5E818]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography from ascites.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).
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