Human Transforming Growth Factor beta 1 from platelets.
TGFb1 (Transforming Growth Factor-beta1) is a family of multifunctional 25kD proteins. TGFb1 was originally identified for its ability to induce the growth of normal rodent fibroblasts in soft agar. It is now known that TGFb1 is a potent growth inhibitor of many normal and transformed cell lines. It regulates normal cell growth, development and tissue remodeling following injury. TGFb1 is produced as latent high molecular weight complexes. The activation of latent TGFb1 is an important step in the regulation of its action. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Membrane permeabilization is required. Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Has been successfully used on FFPE tissue without pre-treatment. Can use citrate, pH 6, EDTA, pH 8, or pepsin mediated antigen retrival. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Immunohistochemistry Postivie Control: Human Breat Carcinoma Hybridoma: SP2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
