Partially purified, large latent transforming growth factor beta 1 complex from human platelets.
TGF-b is synthesized as high molecular weight latent complexes. In platelets, the large latent TGF-b1 complex is composed of three components: mature TGF-b1 dimer, latency associated peptide, and latent TGF-b binding protein (LTBP). The cDNAs for four binding proteins (LTBP-1,-2,-3 and-4) have now been cloned. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with partially purified, large latent transforming growth factor beta 1 complex from human platelets. Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.
