Synthetic peptide corresponding to residues near the C-terminus of human BIG-H3.
BIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683aa secretory protein induced by TGF-B that plays a role in cell adhesion, differentiation, and apoptosis (1-4). BIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM BIG-H3 is found in a wide variety of tissues (8-12). Mutations in the BIG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:800 Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
