Treponema pallidum (Syphilis) ELISA Kit, BioAssay(TM)

Catalog Number: USB-T8300-14
Article Name: Treponema pallidum (Syphilis) ELISA Kit, BioAssay(TM)
Biozol Catalog Number: USB-T8300-14
Supplier Catalog Number: T8300-14
Alternative Catalog Number: USB-T8300-14-1
Manufacturer: US Biological
Category: Kits/Assays
Application: ELISA
The Treponema pallidum (Syphilis) ELISA Kit, BioAssay(TM) is an in vitro enzyme immunoassay for the detection of Anti-TP antibodies in human serum or plasma. It is for diagnosis of early infection and epidemic survey. Assay Principle: This kit uses enzyme immunoassay method to detect anti-TP antibodies in serum or plasma. The recombinant TP antigen TP47 and TP is coated on the multi-wells plate. When serum sample and another recombinant TP antigen labeled with HRP (conjugated) are added to the coated wells, and if Anti-TP antibody is present in the sample, a complex of TPAg-Anti-TP-TPAg labeled with HRP will form. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of Anti-TP antibodies in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of TP infection. Kit Components: T8300-14A1: Microtiter Strips, 1x96 wells T8300-14A2: Sample Diluent, 1x6ml T8600-14A3: Negative Control, 1x1ml T8600-14A4: Positive Control, 1x1ml T8600-14A5: Wash Buffer, 20X, 1x50ml T8600-14A6: HRP Conjugate, 1x12ml T8600-14A7: Substrate A, 1x6ml T8600-14A8: Substrate b, 1x6ml T8600-14A9: Stop Solution, 1x6ml Storage and Stability: Store all components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.