TRIM (T Cell Receptor-Interacting Molecule), IgG1, Clone: [9H187], Mouse, Monoclonal
Biozol Catalog Number:
USB-T8464-35C
Supplier Catalog Number:
T8464-35C
Alternative Catalog Number:
USB-T8464-35C-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
FC, WB
Immunogen:
Recombinant human TRIM, aa50-186, Q6PIZ9 (E. coli).
Human TRIM (T cell receptor-interacting molecule) (also trat1, T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 30kD, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family. It contains a short, 8aa extracellular region, a 19aa transmembrane region, and a 159aa cytosolic tail. Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins. TRIM is present in T cells and NK cells. Human TRIM shares 66% aa sequence identity with mouse TRIM. Applications: Suitable for use in Flow Cytometry, ELISA, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml ELISA: 0.5-1.0ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
