A synthetic peptide from the C-terminus of aa-LNSLNQAVVSKLA.
Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:16,000. Western Blot: 1-3ug/ml. A band is detected at 48kD in Human Brain, Mouse Brain lysates and in lysates of Mouse putuitary cell line NIH3T3 (calculated MW of 51.6kDa according to NP_004227.1). Immunohistochemistry: 3-5ug/ml In paraffin embedded Human Thyroid Gland shows nuclear and cytoplasm staining in activated epithelial cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See T8493-02C-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
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