Purified Succinyl Triticum vulgare lectin (WGA), FITC conjugated. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: (GlcNAc-b(1,4)GlcNAc) 1-4>b-GlcNAc Inhibitory Carbohydrate: N-Acetylglucosamine or its dimers. FITC/Protein Ratio (OD495/OD280): 2.50 Activity: 10ug/ml will agglutinate human erythrocytes nonspecifically. Note: Succinyl WGA does not bind Sialic Acid. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Purity:
Purified
Form:
Supplied as a liquid in PBS, pH 7.2-7.4, 0.05% sodium azide. Labeled with FITC.
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