Treatment of trypsin with phenylmethylsulfonyl fluoride (PMSF) followed by a strong base leads to the conversion of Serine 195 to dehydroalanine at the active site (1). Alpha and beta forms of anhydrotrypsin are separated by immobilized soybean trypsin inhibitor. The activity of anhydrotrypsin is greatly reduced compared to native trypsin as quantified by chromogenic substrate (2). Anhydrotrypsin provided by Molecular Innovations is the single chain beta-form prepared from Type XIII bovine trypsin. It has a MW of 23.3kD and an extinction coefficient of 1.54mg-1 ml-1. Source: Anhydrotrypsin, from bovine pancreas Molecular Weight: 83kD Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
83
Purity:
~95% (SDS-PAGE)
Form:
Supplied as a liquid in 1mM HCl, 10mM CaCl2.
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