TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR) (Biotin), Rabbit

Product Overview

• Article Name: TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR) (Biotin), Rabbit
• Biozol Catalog Number: USB-T8663-BIOTIN
• Supplier Catalog Number: T8663-Biotin
• Alternative Catalog Number: USB-T8663-BIOTIN-100
• Manufacturer: US Biological
• Host: Rabbit
• Category: Antikörper
• Application: IHC, IP, WB
• Immunogen: Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.

Product Description

TrkA has an apparent molecular weight 140kD and is a high affinity nerve growth factor (NGF). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Immunocytochemistry: Positive staining for TrkA in PC-12 cells fixed with 4% paraformaldehyde. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Product Properties

• NCBI: 021589
• UniProt: P35739
• Purity: Purified by Protein A affinity chromatography.
• Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.