TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR) (FITC), Rabbit
Catalog Number:
USB-T8663-FITC
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| Article Name: | TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR) (FITC), Rabbit |
| Biozol Catalog Number: | USB-T8663-FITC |
| Supplier Catalog Number: | T8663-FITC |
| Alternative Catalog Number: | USB-T8663-FITC-100 |
| Manufacturer: | US Biological |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IHC, IP, WB |
| Immunogen: | Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor. |
| TrkA has an apparent molecular weight 140kD and is a high affinity nerve growth factor (NGF). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Immunocytochemistry: Positive staining for TrkA in PC-12 cells fixed with 4% paraformaldehyde. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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