Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3), IgG1, Clone: [1.BB.131], Mouse, Monoclonal
Biozol Catalog Number:
USB-T8665-13C
Supplier Catalog Number:
T8665-13C
Alternative Catalog Number:
USB-T8665-13C-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
ELISA, IHC, IP, WB
Immunogen:
Native troponin complex.
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place Because of which myosin can not bind the actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confirmational changes which leads to dislocation of troponin I and finally tropomyosin leaves the the binding site for myosin on actin leading to contraction of muscle. The letter I is given due to its inhibitory character. Applications: Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry, Immunoaffinity purification and Western Blot. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[1.BB.131]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography from ascites. (>90% SDS-PAGE).
Form:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
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