Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3) (MaxLight 650), IgG2b, Clone: [2Q1100], Mouse, Monoclonal
Biozol Catalog Number:
USB-T8665-13F-ML650
Supplier Catalog Number:
T8665-13F-ML650
Alternative Catalog Number:
USB-T8665-13F-ML650-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
FLISA, IHC, WB
Immunogen:
Bovine cardiac Troponin I.
MaxLight(TM)650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor(TM)647, DyLight(TM)649, Cy5(TM) and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm), Emission (676nm), Extinction Coefficient 250,000. Cardiac Troponin T, a 36kD protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion oncentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms. Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit Troponin C (TnC), the tropomyosin-binding subunit Troponin T (TnT), and the inhibitory subunit Troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place Because of which myosin can not bind the actin in relaxed muscle. When calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of Troponin I. The letter ''I is given due to its inhibitory character. Applications: Suitable for use in Immunohistochemistry, FLISA, Western Blot and Affinity Purification. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
