Troponin I, Cardiac, aa169-184 (cTnI), IgG2a, Clone: [7E149], Mouse, Monoclonal
Biozol Catalog Number:
USB-T8665-16S
Supplier Catalog Number:
T8665-16S
Alternative Catalog Number:
USB-T8665-16S-1
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
WB
Immunogen:
Free human cardiac troponin
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter ''I is given due to its inhibitory character. The tissue specific subtypes are: Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) Cardiac troponin I, TNNI3 (19q13.4, 191044) Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[7E149]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
* VAT and and shipping costs not included. Errors and price changes excepted
