Troponin I, cardiac muscle, PHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1) (MaxLight 550), IgG2b, Clone: [1G11], Mouse, Monoclonal
Biozol Catalog Number:
USB-T8665-18M-ML550
Supplier Catalog Number:
T8665-18M-ML550
Alternative Catalog Number:
USB-T8665-18M-ML550-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
FLISA, WB
Immunogen:
Phosphorylated form of human cardiac Troponin I.
MaxLight(TM)550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor(TM)546, 555, DyLight(TM)549 , Cy3(TM), TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm), Emission (575nm), Extinction Coefficient 150,000. Troponin I is a 24kD subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts. Applications: Suitable for use in FLISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: For the detection of phosphoproteins, phosphatase inhibitors such as 10mM sodium fluoride and 1mM sodium orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[1G11]
Isotype:
IgG2b
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight(TM)550.
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