Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, TNNC1, Troponin I Type 3 Cardiac, TNNI3), IgG1, Clone: [14e1], Mouse, Monoclonal

Product Overview

• Article Name: Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, TNNC1, Troponin I Type 3 Cardiac, TNNI3), IgG1, Clone: [14e1], Mouse, Monoclonal
• Biozol Catalog Number: USB-T8665-20B
• Supplier Catalog Number: T8665-20B
• Alternative Catalog Number: USB-T8665-20B-1
• Manufacturer: US Biological
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Immunogen: Human Cardiac Troponin I (cTnI) (24-40 region)

Product Description

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter ''I is given due to its inhibitory character. Tissue-specific Troponin Subtypes: Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) Cardiac troponin I, TNNI3 (19q13.4, 191044) Applications: Suitable for use in ELISA., Immunoaffinity Purification, and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Matched Pair: Suitable for use as the detection or conjugate (label) antibody. Matched to T8665-20A as the capture or solid phase antibody. Hybridoma: Sp2/0 myeloma cells with spleen cells of Balb/c mice Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [14e1]
• Isotype: IgG1
• Purity: Purified by Protein A affinity chromatography from ascites.
• Form: Supplied as a liquid in 20mM sodium phosphate, pH 7.2, 150mM sodium chloride, 0.09% sodium azide.