Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure. Applications: Suitable for use in ELISA, Western Blot and Immunoaffinity Purification. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Recommended Pair: Suitable for use as the capture or solid phase antibody. Matched to T8665-22B as the detection or conjugate (label) antibody. Also suitable for use as the detection or conjugate (label) antibody. Matched to T8665-22C as the capture or solid phase antibody. Hyrbidoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[1.BB.154]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
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