The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle (TNNT1), cardiac muscle (TNNT2),and fast skeletal muscle (TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2-4ug/ml Immunohistochemistry (Formalin fixed paraffin, formalin or acetone fixed frozen): 4-8ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[10K379]
Isotype:
IgG1
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml PBS.
* VAT and and shipping costs not included. Errors and price changes excepted
