The tyrosinase family in vertebrates consists of three related melanogenic enzymes: tryrosinase, tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2). These proteins control melanin production in pigment cells and play a crucial role in determining vertebrate coloration and are expressed by melanoma cells, normal melanocytes and nevi, but not carcinomas or sarcomas. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Positive Control: SK-MEL-23, A375 cells, Melanoma and Skin Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[3F388]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
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