The tyrosinase family in vertebrates consists of three related melanogenic enzymes: tryrosinase, tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2). These proteins control melanin production in pigment cells and play a crucial role in determining vertebrate coloration and are expressed by melanoma cells, normal melanocytes and nevi, but not carcinomas or sarcomas. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Positive Control: SK-MEL-23, A375 cells, Melanoma and Skin Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[3F388]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
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