Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors. Used in protein sequencing and tissue disassociation. CAS No: 9002-07-7 Source: Porcine pancreas Purity: Chromatographically-purified. SDS-PAGE. Certified to be free of porcine parvovirus. Appearance: Off-white to beige crystalline powder Trypsin: 250u/mg Chymotrypsin: 75u/mg Endotoxin: 10EU/mg Porcine Parvovirus (Hemagglutination, PCR): None Detected Ash: 2.5% Moisture: 5.5% Microbiological Analysis: Plate Count: 1000cfu/g Yeast/Mold: 10cfu/g P. aeruginosa: None Detected in 25g Salmonella: None Detected in 25g E. coli: None Detected in 25g S. aureus: 10cfu/g Unit Definitions: Trypsin: The amount of enzyme which causes an increase in absorbance at 253nm of 0.003 per minute at 25C Chymotrypsin: The amount of enzyme which causes an decrease in absorbance at 237nm of 0.0075 per minute at 25C. Reconstitution: Stock solutions of trypsin should be made initially by reconstituting in 0.001N HCL. This solution can be diluted in the digestion medium or buffer immediately prior to use. The optimum pH of Trypsin is approximately 8.0. Storage and Stability: Powder may be stored at 4C. Stable for 12 months after receipt at 4C. Country of Origin: USA
CAS Number:
[9002-07-7]
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