Trypsin, Porcine, Recombinant, 3800USP unit/mg, CAS [[9002-07-7]]

Product Overview

• Article Name: Trypsin, Porcine, Recombinant, 3800USP unit/mg, CAS [[9002-07-7]]
• Biozol Catalog Number: USB-T8671-10A
• Supplier Catalog Number: T8671-10A
• Alternative Catalog Number: USB-T8671-10A-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA

Product Description

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin lies between 7-9. The enzyme is inhibited by serine protease inhibitors (e.g. PMSF) and metal chelating agents (e.g. EDTA). Recombinant Porcine Trypsin is a genetically engineered protein that is expressed in E. coli and purified by high pressure liquid chromatography. There is no contaminating enzyme activity from carboxypeptidase A or chymotrypsin. No protease inhibitors such as PMSF are used during the manufacturing process. Source: E. coli (Porcine pancreas) E.C: 3.4.21.4 Appearance: White lyophilized powder Purity: 99% (HPLC) Molecular Weight: 23.8kD Protein Content: 70% beta-trypsin, 20% alpha-trypsin Specific Activity: 3800 USP unit/mg protein Unit Definition: One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3ml at pH7.6 and 25°C, with BAEE as a substrate (1cm light path). Contaminant Activity: No chymotrypsin, carboxypeptidase A, or other protease contaminants. Storage and Stability: Store in a sealed container at 2-8C. Once reconstituted, it should be stored at -20C. Stable for 24 months. Greater than 90% enzymatic activity retains after 10 repeated freeze-and-thaw cycles.

Product Properties

• Molecular Weight: 23.8
• Purity: 99% (HPLC)
• Form: White lyophilized powder
• CAS Number: [9002-07-7]