Application(s): suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Recommended Dilution: ELISA: 1:10,000 to 1:100,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form:
Supplied as a lyophilized powder in PBS, pH 7.2, BSA. gentamicin sulfate.
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