Trypsin Inhibitor, Ovomucoid, Western Blot Positive Control (Allergen Gal d I)

Product Overview

• Article Name: Trypsin Inhibitor, Ovomucoid, Western Blot Positive Control (Allergen Gal d I)
• Biozol Catalog Number: USB-T8682-05A
• Supplier Catalog Number: T8682-05A
• Alternative Catalog Number: USB-T8682-05A-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: WB

Product Description

Allergy to chicken egg or proteins is one of the more frequent causes of food hypersensitivity in infants and young children. Both IgG and IgA class antibodies may be detected. Ovalbumin intolerance has been implicated in a number of conditions affecting children. In particular, children with cystic fibrosis show elevated anti-ovalbumin antibodies. Ovalbumin antibodies have also been noted in some forms of kidney disease. A relationship between food allergy and infantile autism has also been observed. Children with insulin-dependent diabetes mellitus show an enhanced immune response to both b-lactoglobulin and ovalbumin, a phenomenon that may be related to the development of the disease. Conditions related to ovalbumin intolerance usually resolve once egg and egg based foods have been withdrawn from the patients diet. Intolerance to egg proteins could be due not only to the ovalbumin protein found in egg white but also to other major proteins present in the yolk. The major proteins of chicken eggs are: Ovalbumin 45kD, 54%), Conalbumin (13%, 80kD), Ovomucoid (11%, 28kD), Lysozyme (3.5%, 14kD), Globulins (G2, G3) (8%), Ovomucin (1.5%). Other protein components include, flavoprotein (0.8%), ovoglycoprotein (0.5%), ovomacroglobulin (0.5%), ovoinhibitor (0.1%) and avidin (0.05%). Chicken trypsin inhibitor or Ovomucoid, OVM, (Allergen Gal d 1) Gal d I) precursor is 210aa protein secreted protein. Ovomucoid consists of three homologous, tandem Kazal family inhibitory domains each homologous to pancreatic secretory trypsin inhibitor Kazal) and each with an actual or putative reactive site for inhibition of serine proteinases. The major reactive site for bovine beta-trypsin is the Arg89-Ala peptide bond in the second domain. it causes an allergic reaction in human. The human antibodies reacted with the carbohydrate-containing domain fragments more strongly than the animal antibodies. Source: Chicken egg ovomucoid protein, purified from egg white. Free from Type-II-O ovoinhibitor. Applications: Suitable for use in Western Blot. Not suitable for use in ELISA or other applications where native protein is required. Other applications not tested. Recommended Dilution: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Purity: Highly Purified (~95%)
• Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.