Tryptase beta-1 (Mast Cell Protease 7) is a serine protease with trypsin-like activity. It is stored in the secretory granules of mouse mast cells. It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma. The deduced amino acid sequence consists of a signal peptide (residues 1-18), a pro region (residues 19-28) and the mature chain (residue 29-273). The mature chain was expressed and purified. Source: Human CD33 Signal Peptide (Met 1- Ala 16) Mouse Tryptase beta-1 (Ile 29-Phe 273). Recombinant mouse Tyrptase beta-1/Mast Cell Protease 7 (rmTryptase beta-1, residues 29-273)1 in the above construct was expressed in a murine myeloma cell line, NS0. Activity: Measured by its ability to cleave a peptide substrate, Benzyloxcarbonyl-Lys-Thiobenzyl ester (Z-K-SBzl), in the presence of 5,5-Dithio-bis (1-nitrobenzoic acid) (DTNB), as measured by absorbance at 405nm and using the extinction coefficient of 13,260 M-1cm-1.2. The specific activity, measured with 100uM Z-K-SBzl and 500ng enzyme in 100ul of 50mM MES, pH 6.5 containing 0.1mM DTNB atRT, is 200pmoles/min/ug. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
