Tryptone from Plant, Animal Free, Atholate(TM)

Product Overview

• Article Name: Tryptone from Plant, Animal Free, Atholate(TM)
• Biozol Catalog Number: USB-T8750-01
• Supplier Catalog Number: T8750-01
• Alternative Catalog Number: USB-T8750-01-100,USB-T8750-01-500,USB-T8750-01-1,USB-T8750-01-2.5,USB-T8750-01-10
• Manufacturer: US Biological
• Category: Zellen/Zellkultur

Product Description

Atholate(TM) is a proprietary blend of plant protein hydrolysates designed to replace traditional casein hydrolysate in the preparation of bacteriological media. Atholate(TM) is APF (Animal-Product- Free) Certified(TM) guaranteed to have no animal derivatives while still maintaining quality performance. Atholate(TM) matches the nutritional and performance characteristics of standard casein hydrolysates and can be used for the cultivation of a wide range of microbial species. Atholate(TM) is the key ingredient in Athenas new Animal-Product-Free LB Broths, which perform equivalent to their animal-derived counterparts. Athenas APF LB Broths are available in Miller, Lennox and Luria formulations and are superb media for the cultivation of Escherichia coli, Salmonella, Pseudomonas, Bacillus and other eubacteria species. In recent years, the use of animal-derived raw materials has become a concern as a source of harmful contaminants to manufacturers of biopharmaceutical products.5 Initially, this concern was confined to the manufacturing end of the industry. As the regulatory environment has evolved in regard to the use of animalderived materials, greater restrictions are being placed at earlier stages of product development. The consequence is a pressing need for media formulations which are not made from animal sources. To address this, we have developed a non-animal protein hydrolysate to replace the casein hydrolysate. An obvious choice for the casein replacement would be plant-derived hydrolysates, however, no one plant protein source has the same amino acid composition as casein. Therefore, the direct replacement of casein hydrolysate with a plant protein hydrolysate would not yield a medium with the same nutritional composition. To overcome this limitation, we have designed a blended plant-based protein hydrolysate that matches casein hydrolysate in composition and in performance. Appearance: Light yellow to yellow, homogeneous, free flowing powder Solubility (20g/L): Tan to light amber, clear, complete pH (2%): As Reported Results and Discussion: Using an in silico model, several candidate blends were devised. The algorithm applied first searched among 30 different plant protein hydrolysates for those with amino acid compositions that most closely matched caseins composition for each individual amino acid. The model then used an iterative process of forming hypothetical blends that resulted in eight possible variations with compositions closely matching casein. These base formulations were then used to derive a mathematical model for optimizing the blending. Figure 1: Growth curves for strain HMS174 cultured in various APF LB formulations. To 1ml of each medium in a 24-well culture dish, a 0.1ml aliquot of an overnight culture grown in LB (Miller) was added. The cultures were incubated in a Tecan Genios microplate reader with shaking at 37C. The absorbance at 600nm was measured at 1 hour intervals. Duplicate wells of non-inoculated medium served as blanks. The net absorbance values were plotted to generate the growth curves. To determine the best formulation, the candidate hydrolysates were blended according to a simplex lattice mixture design.6 Eleven prospective formulations were tested empirically by culturing E. coli strain HMS174 in medium composed of 5g/L yeast extract, 10g/L NaCl and 15g/L blended hydrolysate. Growth was monitored spectrophotometrically over a 24 hour period and the growth rate and yield calculated. Figure 1 shows the results of this initial screen. The growth parameter values were used to construct a mathematical model which to predicted blends that would produce a growth rate and yield that matched the LB reference medium. This model predicted two possible formulations, designated 12 and 13. Two additional formulations, designated 14 and 16, were predicted by examining the compositions that gave the same growth characteristics as the reference medium and selecting compositions intuitively based on this comparis