MaxLight(TM) 490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. Tuberous sclerosis complex (TSC) is an autosomal dominant genetic disorder, which is characterized by seizures, mental retardation and hamartomatous in multiple organs. The genetic disorder is caused by the mutation in two genes - TSC1 and TSC2. The TSC2 gene is located on the chromosome 16p13.3, and it encodes the protein tuberin(1). Tuberin can interact with hamartin, encoded by TSC1 gene, to form a physical and functional complex(2). Tuberin, together with hamartin, are widely localized within epithelial cells, lymphocytes, endocrine glands and central nervous system (CNS) throughout the both normal body and TSC patients(1). Several independent investigations have demonstrated that tuberin is directly phosphorylated by Akt(2,3) and is further involved in stimulating cell growth, which provide an important link between tuberin and growth factor signaling. The loss of tuberin is associated with cell cycle deregulation and tumor suppression(4,5). Applications: Suitable for use in FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[3G293]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight(TM)490.
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