TTK (TTK Protein Kinase, Dual Specificity Protein Kinase TTK, ECSTY Kinase, ESK, FLJ38280, hMPS1, Monopolar Spindle 1 Like 1, MPS1L1, Mps1, Phosphotyrosine Picked Threonine Kinase, PYT), Sheep
Biozol Catalog Number:
USB-T9142-01E
Supplier Catalog Number:
T9142-01E
Alternative Catalog Number:
USB-T9142-01E-100
Manufacturer:
US Biological
Host:
Sheep
Category:
Antikörper
Application:
IHC, WB
Immunogen:
E. coli-derived recombinant human TTK, Ser281-Leu481
TTK (Dual specificity Thr/Tyr kinase, also PYT and hMps1) is a 97-105kD member of the Ser/Thr protein kinase family of enzymes. It is expressed in rapidly proliferating tissues and cell lines, and participates in cell cycle regulation. In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation. Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2. Human TTK is 857 (aa) in length and contains one protein kinase domain (aa525-791). Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686. Apoplications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
