Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1), IgG1, Clone: [DM1A], Mouse, Monoclonal
Catalog Number:
USB-T9154-07
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| Article Name: | Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1), IgG1, Clone: [DM1A], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-T9154-07 |
| Supplier Catalog Number: | T9154-07 |
| Alternative Catalog Number: | USB-T9154-07-200 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IF, WB |
| Immunogen: | Purified human brain a-Tubulin. The epitope recognized by this antibody has been mapped to aa426-450 (AALEKDYEEVGVDSVEGEGEEEGEE). |
| Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-1ug/ml detected a-Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-a-Tubulin (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates a-Tubulin (~50kD). Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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