Acetylated alpha-tubulin from the axoneme of sea urchin sperm flagella.
The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. Stable microtubules (MTs) are known to contain acetylated alpha-tubulin. Acetylated tubulin is found in the dense networks and bundles of microtubules in the sensory and supporting cells. MTs containing acetylated alpha-tubulin in Ni2+-treated cells were resistant to disassembly induced by nocodazole, and at least partially resistant to cold temperature (0 degrees Celsius), which also depolymerizes MTs. Since acetylated alpha tubulin serves as a marker for the presence of stable MTs, the marked enhancement of alpha tubulin acetylation in Ni2+-treated cells indicates that stabilization of MTs may be an important mechanism by which Ni2+ induces cell injury since stabilized MTs in turn should favor MT bundling. The acetylated microtubules have been shown to be more drug resistant than nonacetylated microtubules. This increased stability is most likely due to the acetylation process. Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot (Human, mouse, rat): 1-3ug/ml Immunofluorescence (Human, mouse): 1-3ug/ml Optimal dilutions to be determined by the researcher. Source: Ascites Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[6A205]
Isotype:
IgG2b
Purity:
Purified by Protein A chromatography
Form:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
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