The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. The effects on tubulin messenger RNA levels and tubulin protein synthesis when treating cells with microtubule-depolymerizing drugs or when directly microinjecting cells with tubulin suggest that non-polymerized tubulin depresses its own synthesis. Accumulation of tubulin protein and an increased array of microtubules have been associated with contractile dysfunction in cardiac myocytes after pressure overload in vivo. Studies have also shown that cardiac activity can increase the amount of beta-tubulin in rat cardiac myocytes. Tubulin production in cultured cardiac myocytes can be regulated directly by mechanical forces. In mechanically challenged hearts, the accumulation of beta-tubulin and the development of contractile dysfunction may be directly related to the mechanical forces imposed on the myocardium during the onset and progression of cardiovascular disease. Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot (Human, mouse and rat): 1-3ug/ml Immunofluorescence (C. elegans): 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse NIH3T3 fibroblast cells, rat brain, and mouse testis. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[6A206]
Purity:
Purified by Protein A affinity chromatography
Form:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
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