b-III Tubulin, also known as tubulin b-4, is regarded as a neuron-specific marker. The expression of b-III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium. Applications: Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml with immunofluorescence detection. Used in immersion-fixed rat cortical stem cells and immersion-fixed rat cortical neurons. Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[TuJ-1]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form:
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
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