Tubulin is a major cytoskeleton component that has five distinct forms. a and beta Tubulins form heterodimers which multimerize to form a microtubule filament. There are five beta Tubulin isoforms (beta1, beta2, beta3, beta4A and beta4B) that are expressed in mammalian tissues. beta1 and beta4 are present throughout the cytosol, beta2 is present in the nuclei and nucleoplasm, and beta3 is a neuron-specific cytoskeletal protein. Applications: Suitable for use in Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[10K382]
Isotype:
IgG1
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
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