TBCA (Tubulin folding cofactor A), Clone: [AT1A5], Mouse, Monoclonal

Product Overview

• Article Name: TBCA (Tubulin folding cofactor A), Clone: [AT1A5], Mouse, Monoclonal
• Biozol Catalog Number: USB-T9157-32
• Supplier Catalog Number: T9157-32
• Alternative Catalog Number: USB-T9157-32-100
• Manufacturer: US Biological
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, WB
• Immunogen: Recombinant protein corresponding to aa1-108 from human TBCA, expressed in E. coli.

Product Description

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence (IC): 1,100 Flow Cytometry: 2-5ug/ml to label 1x10e6 cells Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [AT1A5]
• NCBI: 004598
• UniProt: O75347
• Purity: Purified by Protein G affinity chromatography.
• Form: Supplied as a liquid In PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.