Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)
Biozol Catalog Number:
USB-T9160-02A
Supplier Catalog Number:
T9160-02A
Alternative Catalog Number:
USB-T9160-02A-20
Manufacturer:
US Biological
Category:
Molekularbiologie
TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-alpha demonstrate significant cross-species reactivity. TNF-alpha exists in two forms, a type II transmembrane protein, and a mature soluble protein. The TNF-alpha transmembrane protein is proteolytically cleaved to yield a soluble, biologically active, 17kD TNF-alpha, which forms a non-covalently linked homotrimer in solution. Recombinant rat TNF-alpha is a soluble 157 amino acid protein (17.3kD) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. Biological Properties: Recombinant rat TNF alpha was tested in a cytotoxicity assay on murine L929 cells-this batch demonstrated an ED50 of <0.5ng/ml. Specific Activity: 2x10e7 units/mg. Amino Acid Sequence: MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL Storage and Stability: Lyophilized and reconstituted products may be stored at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
